The microscopic detection of animal proteins in feeds

Leo WD. van Raamsdonk; Jeroen Vancutsem; Jos Zegers; Geneviève Frick; Jan-Sten Jorgenson; Victor Pinckaers; Jaume Bosch; Inge Paradies-Severin

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Biotechnologie, Agronomie, Société et Environnement/Biotechnology, Agronomy, Society and Environment

1370-6233 1780-4507

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Leo WD. van Raamsdonk, Jeroen Vancutsem, Jos Zegers, Geneviève Frick, Jan-Sten Jorgenson, Victor Pinckaers, Jaume Bosch & Inge Paradies-Severin

The microscopic detection of animal proteins in feeds

(Volume 8 (2004) — Numéro 4)

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Abstract

In the framework of the European Union funded research project STRATFEED for detection and identification of animal proteins in feeds, the microscopic method was optimized at several key steps and better documented. A check list was developed for uniform reporting. Characters of bone fragments, hairs, muscles and gills are fully documented. A so-called muscle ratio has been developed for the identification of muscle fibers at the level of vertebrate classes (mammals, birds and fishes). Both the improved protocol and the entire range of characters which can be observed, are documented in a Decision Support System called ARIES (Animal Remains Identification and Evaluation System). A second internet-based system called STRATFEED-DSS exclusively assists in identification of animal particles in feeds. A new strategy with microscopy as screening technique and either DNA or protein identification as confirmation technique is proposed. The advantages of this combination are the extremely low level of false negatives, low detection limits and the heat-resistant nature of microscopic detection, together with the possibility of a very specific identification of particles by one of the other methods.

Keywords : animal proteins in feed, ARIES, decision support system, microscopy, muscle ratio

Om dit artikel te citeren:

Leo WD. van Raamsdonk, Jeroen Vancutsem, Jos Zegers, Geneviève Frick, Jan-Sten Jorgenson, Victor Pinckaers, Jaume Bosch & Inge Paradies-Severin, «The microscopic detection of animal proteins in feeds», BASE [En ligne], Volume 8 (2004), Numéro 4, 241-247 URL : http://popups.ulg.be/1780-4507/index.php?id=14150.

Over : Leo WD. van Raamsdonk

Institute of food safety (RIKILT). P.O. Box 230. NL-6700 AE Wageningen (The Netherlands). E-mail: Leo.vanraamsdonk@wur.nl

Over : Jeroen Vancutsem

Agence fédérale pour la sécurité de la chaîne alimentaire – Federal feed and food laboratory (AFSCA-FVLT) of Tervuren. Leuvensesteenweg, 17. B-3080 Tervuren (Belgium).